Özet:

Exposure to asbestos minerals has been associated with a wide variety of adverse health effects including lung cancer, malign pleural mesothelioma, and cancer of other organs. There are asbestos deposits in many regions of Turkey. People in the village of Doğanlı (one of those regions) have been exposed to asbestos all their lives. These people’s Nuclear Division Index (NDI) rations in their lymphocytes were evaluated. Peripheral blood samples were taken from 30 subjects who live in Doğanlı village, age range 30-70, from each gender. Twenty-five people of the same age, sex and socioeconomic group from a village near Kayseri were used as a control group. Blood samples were cultured in Ham’s F-10 medium, which consists of 25% fetal calf serum and phytohemaglutinin. At 44th hour for binucleated cells, Cytochalasin-B (Cyt-B) was added to cultures. After incubation of 72 hours, the slides were prepared according to routine methods. One thousand mononucleated cells were scored for the NDI rations by microscope on slides. At the same time each cell that has two nucleus, three nucleus and four nucleus (binucleated, trinucleated and tetranucleated) were scored and the NDI rations were calculated. In this study, there were significant differences between the NDI values of people who were exposed to asbestos and people in the control group (p=0.001). As a result, these significant differences between the NDI values support that exposure to asbestos is associated with lung cancer and malign pleural mesothelioma (MPM)

Anahtar Kelimeler: