Abstract:

In this study, Listeria spp were isolated and identificated in faecal samples from 100 healthy cows in a Private Commercial Dairy Products Company in different periods (January, October, April, July) of the 2003. For the isolation of Listeria spp., Listeria Selective Broth (Oxoid) and Listeria Selective Agar (Oxoid) were used on the samples of faeces. Upon the first isolation of the samples, the method of cold enrichment was used for the samples on which isolation was not possible. Then the isolation procedure was reapplied to negative samples after the third and the seventh week. In order to grow isolates common biochemistry tests were applied and API Listeria (Biomerieux, France) Test Kit was used. A hundred faeces samples were taken from healthy cattle in the months of January, October, April and July. In the first isolation studies carried out in October, out of 100 faeces, 52 (%52), in January 55 (%55), in April, 31 (%31) and in July 30 (%30) were positive for Listeria spp. In the cold enrichment process, no production of Listeria spp was observed in the 3rd week. In the seventh week, seven (%14.58) of 48 negative samples in October, six (%13.31) of 45 negative samples in January, seven (%11.59) of 69 samples in April, six (%8.57) of 70 negative samples in July were isolated Listeria spp. According to the findings in our research, the spreading, to the nature in the cow faeces of Listeria spp. which has a wide range of survival temperature, is a potantial danger for the health of humans and animals

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