Abstract:

Cervical cancer is one of the most tumors seen in women and the second leading cause of cancer death in women. Cisplatin (CDDP), a platinum-based compound, is the most important chemotherapeutic agent and effectively used for the treatment of sarcomas and solid tumors. It binds to the DNA with crosslinks and leads inhibition of replication, causing DNA damage. As a result of this action, apoptotic pathways are induced and cell death occurs. Methods: In this study, HeLa cells were treated with different concentrations of CDDP at 24 and 48 hours. Cell viability was determined by XTT method. Moreover, after treatment with selected doses of cisplatin, quantitative mRNA expression of mTOR, ACT, CCND1 and STAT-3 genes was analyzed using Real-Time PCR. Results: IC50 concentration of CDDP was found to be about 60 μM for 24h and 8 μM for 48h treatment. Moreover, all analyzed genes' expression was shown to diminish only after 24 h treatment. On the other hand, no statistically significant change was found after 48 h cisplatin exposure with regard to quantitative mRNA expression. Conclusion: In our study, different mRNA expression patterns were found after CDDP treatment regarding to exposure time. Our study has been contributed to literature in terms of detecting the effect of conventional chemotherapeutic CDDP on cell survival pathways.