Abstract:

Purpose: Endotelin (ET-1) is a strong vazo-constructor role in the cardiovascular system as well as manifests itself in many physiological events. It is believed that the ET-1 expression is an important function for both breastfeeding and newborns, especially because it occurs during pregnancy and breastfeeding. In this study, the aim was to study the effects of ET-1 on the breast using the ET-1 transgenic mouse model. A comparative study was conducted to study the effect of ET-1 in different physiological conditions of the breast tissue (adolescence, pregnancy, lactation and involusion). Parallel cuts from the breast tissues were prepared and microscopic examination was carried out with hematoxylene-eosine (HE) painting. The Western blot technique was used in the analysis of signal proteins (STAT5, ACT, STAT3) which have important tasks in breast development and non-metabolism. Genes known to have a key role in metabolic activities (WAP, beta-casein, GLUT-1, SRBF-1 Claudin 8, IL-6, LIF) were analyzed using the real-time polymerase chain reaction (RT-PCR) technique. Results: Patological findings such as secretional damage, lactational insufficiency and early involusion were identified in ET-1 transgenic mice, respectively. In the transgenic group, the activation of STAT5 was not affected by this condition, but there was a decline in the ACT protein levels. STAT3 activation observed in the inflation was detected in transgenic mice on the 3rd day of lactation. Furthermore, RT-PCR analyses have found that the high ET-1 expression in transgenic mice is associated with the ol-rack WAP being pressed and the LIF protein expression has increased significantly. Result: It is believed that ET-1 expressed only during pregnancy and breastfeeding is an important function for breastfeeding and newborns. The high expression of ET-1 has caused laktational insufficiency by directly affecting the synthesis of genes known to be important in breast physiology. This study found that ET-1 regulation plays a critical role in the development of the breast gland and the function of milk secretion.

Keywords: