Abstract:

Campylobacteriosis is an infectious, zoonotic infection characterized by offspring and infertility, leading to economic losses in cattle and sheep breeding. In this study, we aimed to investigate the incidence of cattle and sheep abortion in Kars region in terms of Campylobacteriosis and evaluate the results by PCR, immunohistochemical, histopathological and microbiological methods. In this context, liver and lung tissue examples and abomasum contents of 444 abort cases brought from Kars Center and districts to Kafkas University Faculty of Veterinary Medicine Pathology Department between 2013-2019 years were examined. Tissue examples from animals were fixed in % 10 buffered formaldehyde solutions. After routine procedures, paraffin blocks were prepared and sections with a thickness of 5 μm were taken for Hematoxylin & Eosin staining and 4 μm were taken for immunohistochemical staining . Sections were examined under light microscope to determine histopathologic changes. Organs belonging to aborted fetuses and abomasum contents were inoculated into the Preston Campylobacter Enrichment Broth containing microbial study selective supplement, and then enriched by pre-enrichment and then passed through Preston Campylobacter Selective Agar. Cultures in which the culture was incubated after incubation were examined for colony morphology and microscopic appearance and Campylobacter spp. suspicious colonies were evaluated by biochemical tests. As a result of histopathologic studies, characteristically, 7 of 17 abortion cases with multifocal necrotic hepatitis pattern and yellow abomasum contents were blurred and clotted, PCR, immunhistochemical and microbiological methods detected as Campylobacter spp. positive towards the direction. As a result, we thought that Campylobacteriosis is an important place in the abortion cases from Kars region and should be taken into consideration in breeding.

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