Özet:

Our study aims to identify the penicillium species isolated from the forest soil, through morphological, colonial and molecular methods, and the diversity of penicillium species was studied.Soil samples were taken from the Edirne Soil Forest in October 2015, in aseptic conditions of 10 cm deep, and was used by Waksman's 'Soil Irrigation Method' for the isolation of penicillium species; various works were used for morphological and colonial identification.Bio-Speedy fungal DNA isolation kit is used for molecular diagnosis purposes.™As a PCR kit, Bio-SpeedyTM Maya and Cool Real-Time PCR Fast Detection Kit are used. The resulting sequences were analyzed with similar sequences using the gene bank and the philogenetic tree was drawn using the Mega 7 software. 12 species of penicillium are defined, these are: P. chrysogenum, P. citrinum, P. commune, P. manginii (the current name: Penicillium atrosanguineum), P. ubiquetum (new record for Turkey), P. camemberti, P. sanguifluum, P. janczewskii, P. canescens, P. roseopurpureum, P. steckii (the current name: Penicillium citrinum), P. hordei '.

Anahtar Kelimeler:

Özet:

We studied Penicillium species isolated from forest soil and aimed to be detected with morphologically, colonial-molecular methods and species diversity. Soil sample was taken Edirne Söğütlük Forest in October, 2015 and 10 cm deep under aseptic conditions and Waksman’s ‘Soil Dilution Method’ was used for the isolation of Penicillium species; some books and articles have been used for morphological and colonial identification. The service was purchased for the molecular diagnosis of fungi and the company used the Bio-Speedy ™ Fungal DNA Isolation Kit for isolation. As PCR Kit, Bio-Speedy™, yeast and mould real-time PCR rapid detection kit was used. The obtained sequences were subjected to BLAST analysis similar sequences using the gene bank and to draw phylogenetic tree, used Mega 7 software. Twelve species belonging to the genus Penicillium were identified; these are: P. chrysogenum, P. citrinum, P. commune, P. manginii (current name: Penicillium atrosanguineum), P. ubiquetum (New record for Turkey), P. camemberti, P. sanguifluum, P. janczewskii, P. canescens, P. roseopurpureum, P. steckii (current name: Penicillium citrinum), P. hordei.