Abstract:

This study examined the role of bovin serum albuminine on cell vitality in the cryopreservation of primary rabbit kidney cell culture and continuous HEp-2 cell cultures. Methods: First, cells were cryopreserved with slow, medium and fast freezing methods. As a substance from cryoprotect, the mixture of dimethyl sulfoxide, glycerol and Rowe was used. The cells were frozen with 5% and 10% cryoprotein and ice cream solutions containing 0, 5, 10, 20 and 30g/mL bovin serum albumin. Results: While the highest cell vitality in experiments is achieved by slow freezing method, this method was found to have a statistically significant increase in the vitality of both the primary rabbit kidneys and the HEp-2 continuous cells, exactly in proportion to the concentration of bovin serum albumin involved in the freezing solution (p<0.001). The highest cell vitality was achieved in a ice cream solution containing 10% dimethyl sulfoxide and 30g/mL albumin. Cell vitality was found at 93%±1.1% for rabbit kidney cells, and 96%±1.7% for HEp-2 cells. There was no statistical difference in cell vitality between the two cultures (p>0.05).

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