Özet:

Nano-sized metals are known as effective antibacterial agents at the point of creating resistance against bacteria. These agents are alternatives of natural origin to struggle drug-resistant bacteria, which pose a serious threat to humanity. Silver nanoparticles (AgNPs) have been proven to have good antimicrobial activity. Biosynthesis of AgNPs is accepted as a safer, lower cost, and more environmentally friendly method compared to other nanoparticle production methods. In this study, silver nanoparticles (eAgNPs) encapsulated with Thymus Vulgaris L. aqueous extract were successfully produced using the biosynthesis method. It is aimed to bring in the antiseptic, antimicrobial, and antioxidative properties of the thyme plant to AgNPs. The optimum plant extract and AgNO3 concentration that is required for eAgNP production were determined by UV-vis spectrophotometer analysis and green synthesis was carried out in line with the ratios found. The synthesized eAgNPs were characterized by UV-Vis spectral analysis, FT-IR, XRD analysis, and TEM analysis. The antimicrobial activity of eAgNPs was investigated using a disc diffusion method against E. coli and S. aureus. It has been confirmed that the encapsulation of biosynthesized eAgNPs with the plant extract was successfully carried out by TEM analysis and spherical-shaped biosynthesized nanoparticles had a size range of 6-70 nm. The presence of plant-derived phytochemicals surrounding eAgNPs was confirmed by FT-IR and the formation of AgNP was analyzed by XRD spectrum. Biosynthesized eAgNPs adsorbed into discs at a concentration of 5 mg/mL showed antimicrobial activity against E. coli and S. aureus with mean inhibition diameters of 20.5 mm and 23.2 mm, respectively. The results introduced that eco-friendly eAgNPs with strong antimicrobial activity can be prepared using Thymus Vulgaris L. plant extract and may create a significant potential in the field of biomaterials.

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