Özet:

Medically important plants represent one of the most important sources of drugs in the treatment of various diseases, including cancer, due to their rich components. The aim of this study was to investigate the content of 17 different phenolic substances of methanol (HNM) and water (HNS) extracts of the aerial parts of the Hyoscyamus niger plant and to evaluate the in vitro antiproliferative activity. For this purpose, the content of 17 different phenolic substances of the extracts was determined by HPLC. Antiproliferative activity was determined by MTT assay against osteosarcoma cell line (U-2 OS), liver cancer cell line (Hep G2) and healthy mouse fibroblast cell (L-929) cell lines. The highest amount of ascorbic acid was determined in the extracts. HNM extract was richer in content than HNS extract. According to the antiproliferative activity results, HNM extract showed the strongest effect, especially against Hep G2. When evaluated in general, it was determined that HNM extract was better in terms of phenolic content and antiproliferative activity. By isolating the active components of plant extracts. It is important to conduct more in vivo and in vitro studies.