Abstract:

In the present study, it is aimed to investigate the effect of intraperitoneal use of chitosan on the prevention of peritoneal adhesion formation in the postoperative period immediately after ovariohysterectomy. A total of 15 female Wistar-albino rats weighing 250-350 gr were used for this researcher. Rats were housed in room temperature (22±2°C) and humidity, 12h light-dark cycles. The rats housed in single cages were fed with standard laboratory food and water. After the rats were anesthetized with appropriate anesthetic methods, ovarium and uterus were removed according to the procedure standard procedures used in routines. Rats were randomly divided into 2 groups. Chitosan groups (n=8) were injected intraperitoneally as a single dose mixing 2.5 mL of physiological saline solution with a dose of 50 mg/kg of chitosan immediately after OVH operation. Rats in the other group (n=7), which were evaluated as the control group, were given only 2.5 mL of saline intraperitoneally different from the first group. Body temperatures were recorded on the day of the operation (day 0) and on the 7th and 14th day following the operation. The animals were euthanized 21 days after the operation with a high dose of anesthetic. There was no difference between the groups in terms of body temperatures. Rat without adhesion was found to be higher in the chitosan group. In addition, adhesion resistance was found to be lower in chitosan group. Especially, the number of adhesions score 3 was higher in the control group. These results show that intraperitoneal chitosan application decreases the adhesion rate and adhesion resistance.

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