Özet:

Aspir (Carthamus tinctorius L.) is an important oil plant that has the ability to be an industrial source of raw materials containing oleic acid and α-linolenic acid from high-quality non-saturated fatty acids in its seeds. Pendimethaline is an herbicide of the dinitroanyline group applied to economically valuable plants to ensure foreign herb control. Salisylic acid is an important plant hormone involved in plant defense against biotic and abiotic stress. Omega-3 fatty acids (FAD3 and FAD7) are key enzymes for α-linolenic acid biosynthesis. This study has studied the potential effects on the levels of genetically described FAD3 and FAD7, which are involved in the metabolism of the fatty acid of pendimethaline and salicylic acid. In the study, a total of 36 spots were applied 0,004 and 0,01 M pendimethaline to aspirin plants grown under controlled climate conditions. 0.05 mM salicylic acid has been applied to the samples applied by Pendimethalin. After application, RNA isolation, cDNA synthesis and quantitative polymerase chain reaction have been performed. The variation of layers between the test and control groups is calculated by the 2^ (delta delta CT) formula. The change between the genetic narrative levels was assessed by the t test (p<0,05). Pendimethaline regulates the FAD3 and FAD7 genes downward, with increased concentration of pendimethaline reducing the level of genetic description. Salisylic acid has been found to have a reductive effect on the toxic effect that pendimethaline reveals, regulating the FAD3 and FAD7 genes upwardly. It is believed that Pendimethaline may cause a decrease in the quality of fatty acid in the Carthamus tinctorius L. Remzibey variety and the biosynthesis of α-linolenic acid, while salicylic acid may have a dissuasive effect on the negative effects of Pendimethaline and may contribute to a partial increase in the amount of α-linolenic acid.

Anahtar Kelimeler:

Özet:

Safflower (Carthamus tinctorius L.) is an important oil plant, which is an industrial raw material source containing high quality unsaturated fatty acids, oleic acid and α-linolenic acid. Pendimethalin is a dinitroaniline group herbicide that is applied to plants of economic value for weed control. Salicylic acid is an important herbal hormone involved in plant defense against biotic and abiotic stresses. Omega-3 fatty acid desaturases (FAD3 and FAD7) are key enzymes for α-linolenic acid biosynthesis. In present study, the effects of pendimethalin and salicylic acid on the gene expression levels of FAD3 and FAD7 involved in fatty acid metabolism were investigated. In this study, 0,004 and 0,01 M pendimethalin were applied to safflower plants grown under controlled climatic conditions in 36 pots. 0,05 mM salicylic acid was applied to the samples where pendimethalin was applied. After application, RNA isolation, cDNA synthesis and quantitative polymerase chain reaction were performed. The fold change between the test and control groups was calculated with the formula 2^ (delta delta CT). The change between gene expression levels was evaluated by t test (p<0,05). It was determined that pendimethalin downregulated FAD3 and FAD7 genes and decreased gene expression levels dependant to pendimethalin concentration. It was determined that salicylic acid had a decreasing effect on the toxic effects caused by pendimethalin and upregulated the FAD3 and FAD7 genes. Pendimethalin is thought to cause a decrease in fatty acid quality and α-linolenic acid biosynthesis in Carthamus tinctorius L. cv. Remzibey, salicylic acid has a negative effect on pendimethalin and contributes to partial increase in α-linolenic acid.