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Amaç:Akut gastroenteritler tüm dünyada özellikle çocuk yaş grubunda daha fazla olmak üzere morbidite ve mortalitenin en önemli nedenlerinden biridir. Gastroenterite yol açan etkenlerinin bilinmesi, etkin tedavi uygulanmasının yanı sıra gereksiz antibiyotik kullanımının önlenmesini sağlamaktadır. Gastroenterite neden olan viral patojenler rotavirüs (RV), norovirüs (NoV), sapovirüs (SaV), adenovirüs (HAdV), astrovirüs (HAstV) ve bocavirüs (BoV)’dur. Bu çalışmada Aydın ilinde akut gastroenteritli hastalarda İBoV sıklığının ve tiplerinin moleküler yöntem ile araştırılması amaçlandı. Gereç ve Yöntem: Çalışmaya Eylül 2013 ile Ekim 2014 tarihleri arasında hastanemize başvuran akut gastroenteritli 44 (%46)’ ü kadın, 52 (% 54)’ si erkek olmak üzere toplam 96 hastaya ait dışkı örneği dahil edildi. İnsan BoV-DNA’sı NS-1 gen bölgesine gen bölgesine uygun primer dizilerinin kullanıldığı real-time PZR yöntemi ile araştırıldı. Bokavirüs tiplendirilmesi tipe özgül real time PZR ve koenfeksiyon varlığı multipleks PZR ile belirlendi. Bulgular: Çalışmada incelenen 96 örneğin 2'sinin (% 2) İBoV pozitif olduğu belirlendi. Pozitif iki örnek, tipe özgül gerçek zamanlı PZR ile İBOV tip 1 olarak bulundu. Diğer bokavirüs tipleri saptanmadı. İnsan BoV tip 1 pozitif hastalar erkek cinsiyetinde ve 0-2 yaş grubundaydı. İki hastada (% 100) RV ile koenfeksiyon gözlendi. Sonuç: Bu çalışma ile akut gastroenteritli hastalarda etiyolojik ajan olarak bokavirüsun da diğer sıklıkla saptanan virüsler gibi gastroenterite neden olabileceği belirlenmiştir. Gastroenteritlerde viral etkenlerin belirlenmesi, gereksiz antibiyotik kullanımının önlenmesini hem maliyeti hem de patojenlerde antibiyotiklere karşı direnç gelişimini azaltacaktır.
Acute gastroenteritis is one of the major causes of morbidity and mortality worldwide, especially in the age group of children. The knowledge of the factors that lead to gastroenteritis ensures effective treatment as well as the prevention of unnecessary antibiotic use. The viral pathogens that cause gastroenteritis are rotavirus (RV), norovirus (NoV), sapovirus (SaV), adenovirus (HAdV), astrovirus (HAstV) and bocavirus (BoV). This study was aimed at investigating the frequency and types of IBoV in patients with acute gastroenteritis in Aydin province by molecular method. The study included a sample of a total of 96 patients with acute gastroenteritis, including women (46 percent) and men (52 percent) from September 2013 to October 2014. Human BoV-DNA was studied by the real-time PZR method where primary series suited to the gen area of the NS-1 gen area were used. The type of typing of the bokavirus was determined by the specific real-time PZR and the presence of co-infection by multipleks PZR. Results: 2 of the 96 examples studied in the study (% 2) found that IBoV was positive. Two positive examples were found as type-specific real-time PZR with IBOV type 1. Other types of bacteria are not identified. Human BoV type 1 positive patients were male and 0-2-year-old. Two patients (100%) were co-infected with RV. The result: This study has found that bokavirus as an etiological agent in patients with acute gastroenteritis can also cause gastroenteritis, like other frequently detected viruses. Detection of viral factors in gastroenterites will reduce both the cost of preventing unnecessary antibiotic use and the development of resistance to antibiotics in pathogens.
Aim: Acute gastroenteritis is the most important causes of morbidity and mortality, especially in children worldwide. To know the factors that cause gastroenteritis enables effective treatment as well as prevent the use of unnecessary antibiotics. Viral pathogens that cause gastroenteritis; rotavirus (HRV), norovirus (HNoV), sapovirus (HSaV), adenovirus (HAdV), astrovirus (HAstV) and bocavirus (BoV). The aim of study was to investigate the frequency and types of HBOV in patients with gastroenteritis by molecular methods in Aydin province. Material and Method: Between September 2013 and October 2014, a total 96 patients who were admitted to our hospital with stool samples were included. Forty four of patients were female and 52 were male. BoV-DNA was investigated by real-time PCR appropriate to NS-1 gene region. The typing of HBoV was determined by type-specific PCR and the coinfection of the other viruses was determined by multiplex PCR. Results: Two (2 %) of the 96 samples were determined to be HBoV positive. Two samples with positive were determined as HBoV type 1 by type specific real-time PCR. Other types of HBoV not detected. The positive patients for HBoV type 1 were 0-2 years old and male gender. Mixed infection with RV were observed in 2 (100%) patients. Conclusion: It was determined that bocavirus as an etiological agent in patients with acute gastroenteritis may cause gastroenteritis like other frequently detected viruses. The identification of viral agents in gastroenteritis will reduce both the cost of preventing unnecessary antibiotic use and the development of resistance to antibiotics in pathogens.
Alan : Sağlık Bilimleri
Dergi Türü : Uluslararası
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