Abstract:

Purpose: Ventilator-related pneumonia (VIP) is a high mortality rate of hospital infection, which develops in patients with invasive mechanical ventilation and is frequently found in intensive care units. In patients diagnosed with pneumonia related to the ventilator clinically, we aimed at determining their sensitivity to bacteria and antibiotics that reproduce in the respiratory material. Methods: In the study, samples of endotrakeal aspirat (ETA) taken from 50 patients diagnosed clinically with ventilator-related pneumonia in intensive care units were added to the 5% blood agar (Oxoid, Hampshire, ENGLAND) and "Eosin Methylene Blue" (EMB) (Oxoid, Hampshire, ENGLAND) agar. The plaques were incubated at 37°C for 18-24 hours; in pure culture, the plaques that reproduce ≥100 000 cfu/ml were included in the work. Identification of isolated factors and antimicrobial sensitivities have been studied using conventional methods and the Phoenix Diagnostic System (Sparks, MD, USA). The findings: in ventilator-related pneumonia cases; Acinetobacter baumannii (A. baumannii) 42%, Klebsiella pneumoniae (K.pneumoniae) 26%, Pseudomonas aeruginosa (P.aeruginosa) 20%, Serratia marcescens (S.marcescens) 4%, 2%, and Streptococcus pneumoniae (S.pneumoiae), Enterobacter spp, Staphylococcus aureus (S.aureus) and Stenotrophomonas maltophilia (S.maltophilia) are isolated. 42 of the A. baumannii isolates were found not all (100%) sensitive to sephtazidim, imipenem, meropenem, piperasilin-tazobaktam and sephepim. by K. In pneumoniae isolates, gentamine sensitivity was 85%, trimetoprim-sulfametaksazole sensitivity was 62%. In P.aeruginosa isolates, a sensitivity of 10% to piperasilin-tazobaktam has been detected. The result: the direction of the antibiotics to be used in empirical therapy according to the microbiological flora and antibiotic sensitivity of the unit, and after the isolation of the factor, the treatment must be modified with narrow spectrum antibiotics according to the result of antibiotic sensitivity.

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