Özet:

OBJECTIVE: This study was aimed to determine the prevalence and antibiotic susceptibilities of thermophilic Campylobacter spp. in commercial layer flocks in Bandirma. METHODS: Nine commercial layer flocks were visited between August 2013 and February 2014, and a total of 362 fresh fecal specimens were collected from chickens. Fecal specimens were inoculated onto the Modified Charcoal Cefaperazone Deoxycholate Agar and incubated at 42 °C for 48 h. Colonies suspected for Campylobacter spp. were identified at species level by morphological and biochemical characters. The antibiotic susceptibilities of the isolates were determined by using the disk diffusion method. RESULTS: As a result of the bacteriological analysis, Campylobacter spp. was isolated from all flocks (100%) and 39,8% of all the analysed samples. Eighty eight of 144 Campylobacter isolates C. jejuni (61,1%) and 56 of 144 Campylobacter isolates C. coli (38,9%) were identified. According to the results of antibiotic susceptibility test, the isolates of C. jejuni and C. coli were found to be resistant to tetracycline at the rates of 46,6% and 50% respectively. Resistance rates to the fluoroquinolones (ciprofloxacin and enrofloxacin) were found to be 62,5% and 27,3% for C. jejuni isolates and to be 82,1% and 71,4% for C. coli isolates, respectively CONCLUSION: The prevalence of thermophilic Campylobacter spp. was determined to be high in commercial layer flocks. Also, it was found that the isolates of C. jejuni and C. coli are highly resistant to tetracycline, ciprofloxacin and enrofloxacin.

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