Özet:

Early diagnosis and demonstration of drug resistance in patients with tuberculosis has become a necessity because of increasing prevalence of infections caused by Mycobacterium tuberculosis. Numerous new methods are being tested for this purpose. In this study it is aimed to compare conventional culture methods and molecular methods which are used for diagnosis of Mycobacterium tuberculosis in clinical samples accepted by mycobacteriology laboratory. A total of 50 clinical specimens sent for diagnosis of tuberculosis to Erciyes University, Medical Microbiology Department, Mycobacteriology Laboratory were processed. These samples were gastric lavage fluid, bronchoalveolar lavage, sputum, abscess, urine, sterile body fluids, cerebrospinal fluid, tissue and biopsy samples. Microbiological examining was performed with Ehrlich-Ziehl-Neelsen (EZN) staining and cultured in L-J(Löwenstein-Jensen) medium by BACTEC MGIT 960 method. Determination of isolated subspecies at species level was studied with BACTEC 460 NAP test (Becton, Dickinson and Company Sparks, Ireland) MGIT 960 TBc ID (Becton, Dickinson and Company Sparks, USA) test. Susceptibility tests were performed with BACTEC MGIT 960. Smear positive rate of studied 50 tuberculosis suspected clinical specimens were 34%, with EZN dyeing. L-J and/or MGIT methods also resulted 64% cultures positive. 50% of culture positive samples were found Mycobacterium complex group and 14% were MOTT bacillus. In this study where culture has been considered the gold standard; susceptibility and specificity of smear microscopy were found 32% and 66%, respectively. There was no significant statistical difference and low level of the compliance between culture and smar findings (cappa:0.04; p>0.05). In this study; 60% of clinical samples were found susceptible to SIRE (streptomycin, isoniazide, rifampin, ethambutol) in drug susceptibility test. 40% of clinical samples were found resistant to at least one or more drugs. In conclusion; in this study Mycobacterium tuberculosis strains were found resistant to streptomycin frequently and also resistant to at least one or more drugs. These are determined that two traditional culture methods which are used for the diagnosis of M. tuberculosis, L-J solid culture system and MGIT 960 liquid culture system must be used together; and MGIT 960 is an easy-to-use and a reliable system for culture and drug susceptibility tests

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