The purpose of this study was to investigate the ninth grade students’ misconceptions and confusions of concepts in the cell unit according to new biology curriculum which was started during 2008–2009 educational year. In the first part of our study, we investigated the ninth grade biology textbook, aspects which may cause misconceptions and conceptual understanding difficulties are found as eight items. In the second part of our study we determined the eight items which have misconceptions. Then, we developed the conceptual understanding test and we applied this test as pre-test and post-test to the twenty five students. We chose the students by random cluster sampling in Izmir Anatolian High School, located in Izmir-Karabaglar. In the results of the conceptual understanding test, our analysis revealed that students had constructed misconceptions.
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