Özet:

Objective: Atorvastatin is a member of the drug class known as statins, used for lowering blood cholesterol. Literature for the assay and impurity analysis of atorvastatin is usually combined with HPLC methods using UV or mass detection system. These methods are usually a gradient chromatographic separation method and a mobile phase containing buffer system using the provided stand out. In this study, an isocratic method was developed for which diclofenac sodium is used as internal standard for the determination of atorvastatin. Method: The separation was carried out on C18 column using acetonitril and orthophosphoric acid (0.01%) (67:33 v/v) with isocratic separation using reversed phase high performance liquid chromatography. Results: The retention times of atorvastatin calcium is 3.770±0.200 min and internal standard diclofenac sodium is 5.266±0.200 min. The method was optimized using a simple mobile phase; validation parameters such as linearity, accuracy, precision and LOD-LOQ were validated in terms. .  Conclusion: The developed method is simple, sensitive and precise. The developed method can be used as a routine analysis for atorvastatin calcium.

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