Abstract:

The screening of morphological and growth characteristics of mycelial collections of medicinal bracket fungus Ganoderma adspersum (Agaricomycetes, Polyporales) of different geographical origins (Armenia, Georgia, Iran) on malt-extract agar (MEA) and potato-dextrose agar (PDA) media for 6 days, as well as in submerged culture (malt-extract, ME, 200 rt min) for 14 days at different temperature (25, 30, 35, 38 °C) was performed. Species-specific mycelial macro- and micromorphological characteristics, such as white, cottony-felt, later chamois, leathery, creamy-lemon-yellowish fast-growing colonies, round-shaped hyphal clamps, hyaline, round-shaped, smooth chlamydospore-like swellings and brownish cuticular cells, numerous tetrahedral crystals were described in agar and submerged cultures. The formation of small, dense and smooth pellets, as well as hyphal swellings during submerged growth was observed. The favorable growth temperature for studied collections of G. adspersum was 25-30 °C. The revealed cultural characteristics will be used for taxonomic identification and quality control of mycelial cultures during their biotechnological cultivation. References Adaskaveg JE, Gilbertson RL (1986) Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex. Mycologia 78:694–705. https://doi.org/10.2307/3807513

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