Özet:

In this study, the presence of complex vertebral malformation (CVM), which is a hereditary disease, in Black Alaca cattle cultivated in the province of Antalya, was studied using allele-specific polymerase chain reaction (AS-PCR). The Mediterranean University, which is a member of the Association of Antalya Damızlık Beef Farmers and a member of the same association, has studied the presence of CVM hereditary disease in a total of 200 individuals belonging to the Ziraat Faculty’s milk livestock enterprise. The total genomic DNA is isolated from the blood samples obtained from Holstein cows. Different primates were used in the area of the SLC35A3 gene in the 395 bch length located on the 3rd chromosome of the cattle genome to determine normal and mutant allels. According to the results of the agarose gel electrophoresis applied after the AS-PCR process, individuals were genotyped. In this study, 7 of the 200 individuals surveyed were CVM carriers.

Anahtar Kelimeler:

Özet:

This study aimed to analyze complex vertebral malformation (CVM) genetic defect in Holstein raised in Antalya province using allele specific PCR (AS-PCR). The presence of CVM mutant allele was investigated in 200 Holstein cows raised by member breeders of Antalya Cattle Breeders Association and raised in Akdeniz University, Faculty of Agriculture. Total genomic DNA was extracted from blood samples obtained from Holstein cows. In the 395 bp length of SLC35A3 gene region of bovine chromosome 3, different primers were used to identify the normal and mutant allele. By using agarose gel electrophoresis, individuals were genotyped after AS-PCR. In this study, 7 of 200 individuals were found as CVM carriers.