Özet:

This study was aimed at identifying and determining the strength of reaction and intensity of the TB-pH 0.5 and combined EU/SO colours in the kidneys identified using different fixations (Cardueli carduelis). The material was used in the ages of 1-2 years of age with 3 female carduelis (cardueli). The sample was taken from the kidney area and was detected in 10% formaldehyde solution (24-48 hours) and buin solution (16-18 hours). Examples were subsequently passed through routine tissue tracking and blocked in the paraffin. Serious cuts of 5-6 μ thickness were taken from the paraffin blocks. For the determination of the general histological structure, the hematoxylene-eosine (H&E) paint, and for the determination of the mast cells and their intensity, the toluidine blue (TB)-pH 0. 5 and combined alcian blue-safranin O (EU/SO) paint was applied. Later these preparations were taken photos of the necessary areas by examining in the light microscope (Leica DM 500). Mast cells were detected in a fairly broad area, including in the medula in the kidney tissue, in the intercityum around the kidney tubes, in the cortex, in the glomerules and in the blood vessels around the intercityum. As a result, it was found that the mast cells in cuts detected in buin solutions and painted with TB-pH 0.5 showed more clearly metacromase. In the EU/SO combined paint application, mostly blue alcian blue (EU) (+) and less red safranine (SO) (+) cells were detected while red-blue EU/SO (+) (micst) mast cells were not found.

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