Özet:

Amylases are enzymes that break down the niches produced by plants, animals and microorganisms. Amilazes produced by microorganisms have the ability to be used in a wide variety of industrial applications such as pharmacy, food, washing products, textiles, paper and paper powder industry. However, there are still restrictions on the isolation of microorganisms that produce amylase. The plant is an important plant used in many areas, primarily in medicine. The purpose of this study is to obtain amilase that has biotechnologically important importance by carrying out the isolation of bacteria from the beneficial lightweight plant. Bacterial insulation is done using the dilution technique. The plant was planted in the roots of the land. Morphological, physiological and biochemical tests were carried out after isolation. 16 S rRNA analysis was performed for the type diagnosis of the microorganism. The bacterial sequence analysis found that it was Bacillus atrophaeus with 864 base pairs. The biochemical tests that were effective in identification found that the bacterium was gram-positive (+), basile and immovable. Catalase, hemolysis and glucose were found to have positive (+) results; biochemical tests such as oxidase, H2S and indol were found to have negative (-) results. First of all, the optimization of bacterial reproduction was provided. Important parameters that directly affect the reproduction of bacteria such as time, temperature and pH have been studied. The optimum bacterial production was determined at 72 hours, 30 oC and pH 6.0 respectively. For the purpose of determining the production of strong amylase, bacteria were produced in the nishasted solid food site. After the incubation, the microorganism was found to produce amylase using a false solution. Optimal conditions of production of the determined bacteria in which amylase is synthesized have been identified. The maximum production conditions of α-amilaz from Bacillus atrophaeus were 36 hours, 35 oC and pH 6.0. The enzyme characterization was carried out using the supernatant part of the bacteria produced in optimal conditions. The enzyme temperature and pH were found to show maximum activity at 40 oC and pH 6.0 respectively. The desired bacteria was obtained. Detected and detected bacteria that produce amylase have been determined to have the ability to be used in different biotechnology fields.

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