Özet:

Aim: The aim of this study is to determine the effect of the combined application of the plant-derived compound selenium dioxide and the chemotherapeutic drug paclitaxel (PTX) on the MDA-MB-231 breast cancer cell line. Material and methods: In our study, first of all, the MTT test was performed to determine the semi-maximal inhibitory concentration of selenium dioxide. After the experimental groups were formed as SEL1, SEL2, SEL3, PTX, SEL1+PTX, SEL2+PTX, and SEL3+PTX, apoptosis was demonstrated by the TUNEL method, NF-kB expression was demonstrated by the immunofluorescent staining method, and cell viability was demonstrated by MTT analysis. Results: Selenium dioxide has been shown to decrease cell viability in breast cancer cells. Apoptosis was observed to be increased in SEL1, SEL2, SEL3, and PTX groups compared to control(p<0.001, p<0.001, p=0.312, p<0.001). It was determined that apoptosis was decreased in the SEL1+PTX, SEL2+PTX and SEL3+PTX groups compared to the PTX group (p=0.009, p=0.011, p=0.010). A decrease in NF-kB immunoreactivity intensity was observed in the SEL1, SEL2, SEL3, PTX groups compared to the control (p<0.001, p=0.002, p>0.999, p<0.001). It was determined that there was an increase in NF-kB expression in the SEL1+PTX, SEL2+PTX and SEL3+PTX groups compared to the PTX group (p>0.999, p>0.999, p>0.999). There was a statistically insignificant increase in cell viability in the SEL1+PTX, SEL2+PTX, and SEL3+PTX groups compared to the PTX group. Conclusion: In the current study, it was determined that SEL and PTX have antagonistic effects on the MDA-MB-231 breast cancer cell line.

Anahtar Kelimeler: