Özet:

In this study, the anti-microbial effectiveness of the isolation, identification and anti-microbial effectiveness of lactic acid bacteria in unbranded, unpacked kefir samples presented for sale in the Afyonkarahisar Province was evaluated. From 5 different kefir samples, 6 lactic acid bacteria were isolated and 16S rDNA PZR analysis was performed for molecular identification of insulates. According to the results of the 16S rDNA sequence analysis of isolates, the isolated bacteria are defined as Lactobacillus fermentum, Enterococcus faecium and Enterococcus durans. Lactic acid bacterial insulates have been used to determine the antimicrobial activity on the pathogenic test microorganisms and the agar spot and agar tree diffusion test. All insulates have shown antimicrobial effect at different levels on the patogenic microorganisms tested. Laktic acid bacterial isolates, Klebsiella pneumoniea NRRLB 4420, Pseudomonas aeuroginosa ATCC 11778, Listeria monocytogenes ATCC 1911 had higher antimicrobial effect than other test microorganisms (Staphylococcus aureus ATCC 25923, Streptococcus feacalis, Escherichia coli ATCC 35218, Bacillus subtilis NRS-744) .

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