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Bu çalışmada, borik asitin Penicillium expansum’a karşı etkinliği hem in vitro hem de in vivo koşullarda değerlendirilmiştir. In vitro’da, borik asitin %0.125 konsantrasyonu P. expansum’un misel gelişmesini, spor çimlenmesini ve çim tüpü uzunluğunu sırasıyla %68.88, %74.00 ve %85.50 oranında azaltmış, halbuki daha yüksek konsantrasyonlar (%0.25, %0.50, %1.00 ve %2.00)’ı ise tamamen engellemiştir. Ayrıca borik asitin yukarıda belirtilen ilk konsantrasyonu ve sonraki konsantrasyonlarının etkileri arasındaki fark istatistiksel olarak önemli bulunmuştur (P<0.05). Borik asitin EC50, minimum engelleyici konsantrasyon (MIC) ve minimum fungisidal konsantrasyon (MFC) değerleri sırasıyla, 0.09, %0.25 ve >%2 olarak belirlenmiştir. In vivo denemelerde, borik asitin %0.25 konsantrasyonu hariç, diğer tüm konsantrasyonlar (%0.50, %1.00 ve %2.00)’ı hem koruyucu hem de tedavi edici etki göstermişler ve bu konsantrasyonlar mavi küfün hastalık şiddetini patojen ile inokule edilmiş kontrole kıyasla önemli ölçüde azaltmıştır (P<0.05). Bununla birlikte, %2.00 borik asitin hem koruyucu hem de tedavi edici uygulamalarda patojene karşı en etkili konsantrasyon olduğu ve hastalık şiddetini sırasıyla %94.46 ve %91.41 oranında azalttığı gösterilmiştir. Bu sonuçlar, borik asitin elmalarda P. expansum'un neden olduğu mavi küf hastalığının mücadelesi için sentetik fungisitlere bir alternatif olarak kullanılabileceğini göstermektedir.
In this study, the effectiveness of boric acid against Penicillium expansum was assessed in both in vitro and in vivo conditions. In vitro, the 0.125% concentration of boric acid reduced the similar development of P. expansum, sports grinding and grass tube length by 68.88, 74.00 and 85.50 per cent respectively, while higher concentrations (0.25 per cent, 0.50 per cent, 1.00 per cent and 2.00 per cent) completely prevented. Also, the difference between the first concentration of boric acid mentioned above and the effects of its subsequent concentrations has been statistically significant (P<0.05). The values of boric acid EC50, the minimum inhibitor concentration (MIC) and the minimum fungicidal concentration (MFC) are determined at 0.09, 0.25% and >2%, respectively. In vivo tests, boric acid was 0. Except for 25 concentrations, all other concentrations (%0.50,%1.00 and%2.00) showed both protective and therapeutic effects and these concentrations significantly reduced the severity of the disease of the blue cabbage compared to the inoculated control of the pathogen (P<0.05). However, 2.00% boric acid has been shown to be the most effective concentration against patogen in both protective and therapeutic applications and reduces the severity of the disease by 94.46% and 91.41% respectively. These findings show that boric acid can be used as an alternative to synthetic fungicides for the fight against the disease of the blue cabbage caused by P. expansum in apples.
Efficacy of boric acid against Penicillium expansum was evaluated under both in vitro and in vivo conditions in this study. In in vitro, 0.125% concentration of boric acid reduced mycelial growth, spore germination and germ tube elongation of P. expansum by 68.88%, 74.00% and 85.50% respectively, whereas the higher concentrations (0.25%, 0.50%, 1.00% and 2.00%) of boric acid completely inhibited those of P. expansum. Moreover, the difference among the effects of the above-mentioned first concentration and subsequent concentrations of boric acid was statistically significant (P<0.05). The EC50, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of boric acid were defined as 0.09, 0.25% and >2%, respectively. In in vivo experiments, except for 0.25% boric acid, all the other concentrations (0.50%, 1.00% and 2.00%) exhibited both protective and curative activity against P. expansum, and they significantly reducing the disease severity of blue mold in comparison to pathogen‐inoculated control (P<0.05). However, 2.00% boric acid has been shown to be the most effective concentration against the pathogen in both protective and curative applications, and reduce the disease severity by 94.46% and 91.41%, respectively. These results indicate that the boric acid can be used as an alternative to synthetic fungicides for the control of blue mold disease caused by P. expansum in apples.
Field : Fen Bilimleri ve Matematik; Ziraat, Orman ve Su Ürünleri
Journal Type : Uluslararası
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