Özet:

The blood taken to single use blood bags can be stored up to one week without freezing. Blood bags contain sodium citrate as anticoagulant. The use of defrozen blood in patients can not be beneficial effectively by body. The inner surface of the disposable blood bags was modified with several methods in order to be more effective when used in patient, make blood to stored for longer period, and to prevent the lysis of the blood cells after frozen and defrozen processes. It is clear the the use of blood without freezing will be more effective in healing time of patients. The surface of the commercial blood bags was modified in order to increase the biocompatibility and gain antibacterial property. For this purpose, physical adsorption method wase used to change the single use blood bag’s surface made from medical grade polyvinyl chloride (PVC) that contact with blood. Physical adsorption was selected due to trace materials and toxicity factors coming from conventional chemical adsorption methods and to increase the adsorption capacity. Hence, surface modification processes were conducted with physical adsorption method. In this context, the inner surface of single use blood bags was modified by using three methods. Biomaterial surfaces were modified with physical adsorption method with three different types of arrangments which are selected as PEG, AgNP ve PEG+AgNP. The silver nanoparticles (AgNPs) synthesized with green chemistry approach in our group to attain antibacterial properties. The AgNPs synthesis was performed by liquid leave broth of Muğla region Liquidambar orientalis Mill. (Sığla ağacı) plant. The change in functional groups were determined by FTIR spectrums, the presence of biocompatible material, PEG was also conformed by ith functional groups on biomaterial surface. In order to determine the antimicrobial activities of modified surfaces, the growth of nosocomial microorganisms was estimated by Disk diffusion method. The culturing in Muller Hinton agar containing petri dishes were performed after the activation of bacteria (E. coli, S. aureus, K. pneumoniae, P. aeruginosa, Metisilin dirençli S. aureus) in Nutrient broth, and C. albicans in Saboraud dextrose. Biomaterials with different surface properties were cutted at predetermined size were placed on growth media to incubate and the inhibition zones formed after incubation time were measured. Antimicrobial activity on untreated surfaces was about 50 % while it was calculated. This ratio reached to 89 % in physical adsorption tests. In conclusion, the proposed methods can be used to add antimicrobial activity on biomaterial surfaces againts nosocomial microorganism types.

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