Özet:

Objective: In this study, we examined the anti-inflammatory and antioxidant (ABTS radical scavenging) activity of methanol extracts of aerial parts (except capitula) and capitula of Centaurea cuneifolia, C. iberica, C. kilaea, C. solstitialis subsp. solstitialis and C. stenolepis for the first time comparatively. Materials and Methods: The antioxidant and anti-inflammatory activity, expressed as IC50 values, were determined by 2, 2’- Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic asit (ABTS) and 5-lipoxygenase methods. The total phenolic content, expressed as gallic acid equivalents, was estimated by Folin-Ciocalteu method. Results: Methanol extract of capitula of C. solstitialis subsp. solstitialis (CSSC) with an IC50 value of 8.74 µg/mL showed antioxidant activity as strong as standard acarbose (4.41 µg/mL) against ABTS radicals. The IC50 values of ABTS radical scavenging activities of other extracts varied between 24.42 and 88.95 µg/mL. CSSC with an IC50 value of 122.10 µg/mL displayed moderate inhibitory activity against 5-lipoxygenase enzyme. The IC50 values of the antilipoxygenase activities of the other extracts were found to vary between 122.10 and 781.30 µg/mL. Also, the highest amount of total phenolic compounds was found in the CSSC (83.41 mg/g), while the lowest was found in methanol extract of aerial parts of C. solstitialis subsp. solstitialis (35.20 mg/g). Conclusion: These results clearly indicate that CSSC has significant antioxidant and anti-inflammatory activity. As far as is known, this paper is the first comparative study on ABTS radical scavenging and lipoxygenase inhibitory activity of five different Centaurea species. It is also the first study on the antilipoxygenase activity of C. iberica and C. solstitialis subsp. solstitialis.

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