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  Citation Number 5
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Koyun ve Keçi Sütlerinde Coxiella burnetii Varlığının PCR ile Araştırılması
2017
Journal:  
Veterinary Sciences and Practices
Author:  
Abstract:

Q humması, büyük ölçüde geviş getiren hayvanların süt, vajinal mukus ve dışkı gibi ürünlerle dışarıya saçtığı Coxiella burnetii bakterisinin neden olduğu zoonotik bir hastalıktır. Sığır, koyun ve keçiler etkenin insanlara bulaşmasında primer rezervuar konakçılardır. Bu çalışmada abort yapan ve yapmayan farklı koyun ve keçi sürülerinden toplanan 120 süt örneği, C.burnetii suşunun transpozon repetitive gen bölgesinden türetilen tür spesifik Trans 1 ve 2 primerleri kullanılarak PZR amplifikasyonuna maruz bırakıldı. Elazığ ve komşu illerden toplanan sütlerde abort yapmış koyunlarda %10 ve abort yapmış keçilerde ise %3.33 oranında C.burnetii DNA’sı PZR ile pozitif olarak tespit edildi. Abort yapmayan sürülerden alınan koyun ve keçi sütü örneklerinde PZR testi ile pozitiflik saptanmadı. Sonuç olarak abortus öyküsü olan ve olmayan koyun ve keçi sürülerinden toplanan süt örneklerinde C.burnetii enfeksiyonunun moleküler yöntemlerle araştırılması amaçlanmıştır.

Keywords:

Research of the Presence of Coxiella burnetii in Sheep and Sheep Milk with PCR
2017
Author:  
Abstract:

Q humma is a zoonotic disease caused by the Coxiella burnetii bacteria, which is largely triggered by milk, vaginal mucus, and ejaculation products by animals that bring relief. Cows, cows and goats are primary reservoirs in the infection of the factor to humans. In this study, 120 milk samples collected from different sheep and sheep breeds who aborted and who did not, were exposed to PZR amplification using the specific Trans 1 and 2 primates of the species derived from the transpozon repetitive gen area of the C.burnetii shell. In the milk collected from poor and neighboring regions, 10% of abortion cows and 3.33% of abortion cows had C.burnetii DNA positively identified with PZR. Sheet taken from non-aborted breeds and the positive with the PZR test was not identified in cattle milk samples. As a result, the study of the C.burnetii infection in the milk samples collected from cows and goats, which have a history of abortion and are not, is aimed at using molecular methods.

Keywords:

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Veterinary Sciences and Practices

Journal Type :   Ulusal

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Article : 559
Cite : 1.550
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Veterinary Sciences and Practices