Abstract:

Urtica spp is one of the foreign herbs, which is the most common in the nuts areas and empty lands in the Black Sea Army Province. In this study, Urtica spp.For the purpose of determining the genetic differences of 20 Urtica spp from the Army province. The samples were collected and analyzed using primates specific to the trnL-F gene areas. DNA isolation was carried out by modifying the CTAB (hexadecyl-trimethyl-ammoniumbromide) protocol. For the chloroplast DNA trnL-F gene regions, the sequence results obtained in the study were compared with the reference sequence series provided by GenBank. The genetic distances of the series were calculated using the MEGA6 package program and with the help of these data sets filogenetic tree drawing was provided. Four samples of Urtica (Fatsa 1-U1, Ulubey-U2, Thursday 1-U3 and Altınordu 3-U4) were selected in the area and used in genetic analysis. The study found that all of these samples were found in the same descent as Urtica dioica. Fatsa 1-U1 and Ulubey-U2 samples were seen as close relatives of U. dioica (KF138424) in terms of 99.2% and 99.7% nucleotid series similarity, respectively. These relationships are supported by the algorithm values of 100, 100% and 99%, respectively, found in NJ, MP and ML trees. Nucleotide series similarities between Thursday 1-U3 and Thunderbird 3-U4 samples and U. dioica (AY208725) were found at 99.5% and 100% respectively. The algorithm values of this type are determined in NJ, MP and ML at 67, 64% and 64% respectively.

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