Özet:

INTRODUCTION: In this study, the effects of CCl4 on GSH and GST that have an important role on detoxification reactions, and MDA as diagnostic indices of lipid peroxidation and possible protecting effects of NAS which is precursor of GSH were investigated in the presence of CCl4 induced liver injury. METHODS: In this study, CCl4 was applied a 2 ml/kg (1/1 dissolved in olive oil) ip as a single dose to create liver toxicity. N-acetyl sistein application (inside peritoneum 50/mg/kg/day) was started 3 days before that CCl4 injected to tested group and continued during the experiment. Blood samples and livers tissues were taken by ether anesthesia from 6 hours and 72 hours after CCl4 injection. RESULTS: When tissue antioxidant levels were evaluated, GSH levels were importantly lower than 6th than control group and increased with NAS addition. GST levels were increased in CCl4 given group at 6th hour and 72th hour than control group but the difference at 6th hour was not important (P>0.05). When NAS was given to CCl4 group, It was observed that the falling GST levels at 6th hour were rise at 72th hour. The increased GSH and GST levels and the decreased MDA levels at 6th hour in the given NAS group showed that NAS was very important as antioxidant and this effect diminished by time. The liver GSH and MDA levels at 6th hour in the control group of rats were higher than the findings of 72th hours (P<0.05). This unexpected different in the control group may be due to injection stress or given olive oil. DISCUSSION AND CONCLUSION: As a result CCl4 intoxication was tolerated by organism with time and NAS has beneficial effects on CCl4 caused liver defects. Advanced researches may determine the NAS levels and to consider GSH between GST levels will contribute to study results.

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