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Bu çalışmada, tıbbi aromatik bitkilerden yıllık pelinotunun (Artemisia annua L.) kimyasal kompozisyonu ve antioksidan aktivitesi üzerine sub ve süperkritik akışkan ekstraksiyon metodlarının etkileri incelenmiştir. Uygulanan sub/süperkritik akışkan ekstraksiyonları, subkritik su, subkritik etanol ve süperkritik CO2 kullanılarak gerçekleştirilmiştir. Bu yöntemlerin etkinliği su buharı destilasyonu, geri soğutucu altında kaynatma ve ultrasonik banyoda organik çözücü ile ekstraksiyon yöntemleri kullanılarak kıyaslanmıştır. Ekstraktların antioksidan aktivitelerini belirlemek için serbest radikal temizleme yöntemi (DPPH), toplam antioksidan kapasite (CUPRAC) ve toplam fenol içeriği (FOLIN) yöntemleri uygulanmıştır. Sonuçlar ekstraktlardaki bileşen ve konsantrasyonların ekstraksiyon metoduna göre değişiklik gösterdiğini ortaya koymuştur. Bu farklılıktan yararlanarak ekstraktın kullanım amacına uygun ekstraksiyon metodu seçilebilir. Ekonomik olarak önemli olan oksijenli bileşenler en fazla sub-kritik su ekstraksiyonu ile elde edilmiştir. Antioksidan aktivite belirleme çalışmalarında en etkin yöntemin sub- kritik etil alkol ekstraksiyonu olduğu belirlenmiştir. Elde edilen sonuçların farmakoloji, gıda ve nutrasötik sektörlerine önemli katkı sağlayacağı düşünülmektedir.
In this study, the effects of sub and supercritical fluid extraction methods on the chemical composition and antioxidant activity of annual pelinotine (Artemisia annua L.) from medicinal aromatic plants were studied. Applicated sub/super-critical liquid extractions are made using sub-critical water, sub-critical ethanol and super-critical CO2. The effectiveness of these methods is compared with the use of water steam distillation, cooling under the refrigerator and extract methods with organic solvent in the ultrasonic bath. To determine the antioxidant activities of the extract, the free-radical cleaning method (DPPH), total antioxidant capacity (CUPRAC) and total phenol content (FOLIN) methods have been applied. The results showed that the ingredients and concentrations in the extract varied according to the extraction method. Using this difference, the extraction method can be selected in accordance with the purpose of the extract. The most economically important oxygen components are obtained by sub-critical water extraction. The most effective method in antioxidant activity determination studies has been determined to be sub-critical ethyl alcohol extraction. It is believed that the achieved results will make a significant contribution to the pharmacology, food and nutrition sectors.
In this study, the effects of sub and supercritical fluid extraction methods on the chemical composition and antioxidant activity of annual wormwood (Artemisia annua L.) which is important medicinal aromatic plants were investigated. The applied sub/supercritical fluid extractions were subcritical water, subcritical ethanol and supercritical CO2. The efficiency of these methods has been compared by using steam distillation, refluxing and organic solvent extraction in an ultrasonic bath. Free radical scavenging method (DPPH), total antioxidant capacity (CUPRAC) and total phenol content (FOLIN) methods were conducted to determine the antioxidant activities of the extracts. The results revealed that the components and concentrations in the extracts varied according to the extraction method. Taking advantage of this difference, the extraction method suitable for the purpose of use of the extract can be selected. Economically important oxygenated components were mostly obtained by subcritical water extraction. It was determined that the most effective method in antioxidant activity determination studies was subcritical ethyl alcohol extraction. It is thought that the results obtained will make an important contribution to the pharmacology, food and nutraceuticals industries.
Field : Mühendislik
Journal Type : Ulusal
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