Özet:

Objective: We examined the insertion I and deletion D polymorphism of angiotensin converting enzyme ACE gene polymorphism in 54 children with HSP to investigate the associated with presentation, prognosis and progression of children with Henoch-Schönlein purpura HSP . Materal and Methods: Blood samples were collected from patients and obtained into EDTA. Genomic DNA from peripheral blood lymphocytes was purified. The ACE I/D gene polymorphism was detected by PCR with primer sequences derived. Results: The percent of ACE genotypes II, ID and DD in all patients was detected 4 %, 74 % and 22 % respectively. Patients were divided into 3 groups as HSP nephritis n=14 , HSP with minor urinary anomaly n=18 and HSP without renal involvement n=22 . The distribution of ACE genotypes II, ID and DD respectively in HSP nephritis was 7 %, 57 %, 36 %; in HSP with minor urinary anomaly was 0 %, 89 %, 11 %; and in HSP without renal involvement was 5 %, 72 %, 23 % detected. No association was found between the ACE genotypes and the presence of renal involvement χ2 =4.39, p=0.356 . The distribution of ACE allels I and D respectively in HSP nephritis was 36 % and 64 %; in HSP with minor urinary anomaly was 44 % and 56 % and in HSP without renal involvement was 41 % and 59 % detected. No association was found between the ACE allels and the presence of renal involvement χ2 =0.500, p=0.780 . Conclusion: In conclusion these results does not support a statistically significantly association between renal involvement and DD genotype or D allel in children with HSP. However D allele was detected in 96 % patients and DD genotype was higher in patients with HSP nephritis than patients without renal involvement. According these results we showed that D allel is a facilitative and I allel is a protective factor for development and renal involvement of disease. However larger studies are required to confirm these results.

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