Abstract:

Purpose: This study was aimed at revealing the effects of Galic acid, which is added to the moisturizer in different proportions in the non-season period, in the short-term storage of the coach sperm. Tools and Methods: In the study, a private sheep plant located in Burdur Çallıca was used by 4 heads of Merinos coach (2-3 years old). During the study, sperm was obtained from the coaches with the help of an electro-execulator twice a week. The sperm motility of the collected sperm was 80%>, the density of 0 mM (control) with 1.5x109 sperm/ml were pooled and experimental groups were created. The trial groups were determined in such a way that they would be non-antioxidant (control), 2 mM, 5 mM and 10 mM doses of gallic acid and were stored at +4 C0 respectively. After the creation of the groups, 24 hours and 96 hours were evaluated in terms of subjective motility (%), morphological (%) and membrane integrity (HOS test, %). Results: The study at the end of the 48th hour, the highest motility according to the control group (%51.0±2.7) was obtained from the hydrating groups containing 2 mM (%72.7±5.3) and 5 mM (%69.4±4.8) of gallic acid (P<0.05). At the end of the storage period (96. hours) gallic acid 2 mM and gallic acid 5 mM groups showed more protective properties compared to the sperm motility control group (P<0.05). However, gallic acid showed negative effects according to the control group in terms of membrane integrity of 10 mM. (P < 0.001 ) There was no statistical difference between the groups in terms of anormal sperm rate (P>0. and 05). However, an increase in an abnormal sperm rate was statistically significant depending on the storage time (P <0.001). Recommendation: As a result, the addition of gallic acid in doses of 2 and 5 mM to the moisturizer for the purpose of short-term storage of the coach sperm outside the season has been shown to have a positive effect on the motility rates.

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