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1
A rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method hasbeen developed for simultaneous estimation of tobramycin and dexamethasone.Chromatographic separation was achieved on reverse phase Hypersil BDS C18 column (150 X 4.6 mm,5 µm) using the mobile phase consisting of phosphate buffer pH 6.0 and acetonitrile in the ratio of 70:30, v/v. The mobile phase was pumped at a flow rate of 1.0 ml/min and detection was done by UVdetector at 263 nm. The proposed method was found to be simple, fast, accurate, precise and reproducible and could be applied for routine quality control analysis for simultaneous determination of tobramycin and dexamethasone in pharmaceutical dosage forms.
Alan : Fen Bilimleri ve Matematik; Sağlık Bilimleri
Dergi Türü : Uluslararası
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