Özet:

This study was planned to investigate the possible protective effect of Vitamin C on the ionized radiation inducednecrotic and autophagicpathways as in vitro. The NRK-52E renal epithelial cells were cultured (RPMI 1640 medium(10% FBS. 1% L-Glutamine (2mM) and 1% Penicillin/Streptomycin). The proliferation-enhancing concentration ofVitamin C (100 μM) was used. In this study 5 groups were designed (Control, Ionizing Radiation, Vitamin C, IonizingRadiation+Vitamin C, Vitamin C+Ionizing Radiation). The ionizing radiation groups were exposed 8 Gy ionizing radiation.Total RNA and cDNA were isolated from the samples collected from the incubated cells for 24 hours, andexpressionlevels of target genes were determined by RT-PCR. According to the control gene by RT-PCR; the expression levels ofnecrotic (RIPK 1, RIPK3) and autophagic (ATG3, ATG5, BECN1, Map3A) target genes were determined. In the radiationgroup, necrotic and autophagic genes were found to be significantly increased compared to control group. Thevitamin C supplementation after radiation decreased genes expression of all genes except Map3A gene. As a result, itwas noted that ionizing radiation affects necrotic and autophagic cell pathways in the NRK-52E cell line, and activatecytotoxic mechanism. Vitamin C leads to down regulation for necrotic and autophagic genes, except for Map3Abefore and after ionized radiation administration. It was considered that the effects of vitamin C on cytotoxicity dueto ionizing radiation are worth investigating.

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