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Bu çalışmanın amacı bor nitrür (BN) nanoparçacıklarının floresan bir boya olan rodamin 123 (Rd) ile etiketlenmesi ve biyogörüntüleme amaçlı kullanımının in vitro ortamda araştırılmasıdır. Öncelikli olarak, rodamin 123 etiketi bor nitrür nanoparçacıklarına glutaraldehid çapraz bağlayıcısı aracılığıyla bağlanarak BN-Rd nanoparçacıkları üretilmiş ve SEM, UV-görünür bölge spektroskopisi, MTT hücre proliferasyon testi, ışık mikroskobu ve floresan mikroskobu analizleri ile karakterize edilmiştir. Sitotoksik olmayan konsantrasyonlardaki BN-Rd nanoparçacıkları insan prostat kanseri hücreleri (PC-3) ile insan embriyonik böbrek hücreleri (HEK-293) üzerine uygulanmış ve hücreler floresan mikroskobu altında incelenerek BN-Rd’nin biyogörüntüleme potansiyeli in vitro ortamda araştırılmıştır. Sonuç olarak, SEM analizlerinde ~40 nm boyuta sahip olduğu belirlenen BN ve BN-Rd 30 µg/ml ve üzerindeki konsantrasyonlarda sitotoksik etki göstermiştir. Işık mikroskobu altında incelenen nanoparçacık uygulanmış hücre morfolojileri MTT sonuçlarını destekler nitelikte bulunmuştur. Her iki hücre hattı üzerine de uygulanan BN-Rd’nin 10 µg/ml konsantrasyonda hücreler ile etkileşime geçtiği ve hücrelerin in vitro ortamda floresan mikroskobu altında ışıma yaptığı gözlenmiştir. Bu çalışmadan elde edilen bulgular ışığında rodamin 123 floresan boyası ile etiketli bor nitrür nanoparçacıklarının biyogörüntüleme uygulamalarında kullanım potansiyeli ortaya konmuştur.
The aim of this study is to label bor nitrate (BN) nanoparticles with rodamine 123 (Rd), a floresan paint, and to investigate the use for biodegradation in in vitro environments. Primarily, Rodamin 123 label was produced by BN-Rd nanoparticles by connecting to bor nitride nanoparticles through the glutaraldehyde cross connector and was characterized by SEM, UV-visible area spectroscopy, MTT cell proliferation test, light microscope and fluorescent microscope analysis. BN-Rd nanoparticles in non-citotoxic concentrations were applied to human prostate cancer cells (PC-3) and human embryonic kidney cells (HEK-293) and the cells were studied under a fluorescent microscope and the potential of BN-Rd for biogestion was studied in vitro environment. As a result, in SEM analyses, BN and BN-Rd showed cytotoxic effects in concentrations of 30 μg/ml and above, which were determined to have a size of ~40 nm. The nanoparticles studied under the light microscope have been found to support the MTT results in applicable cell morphologies. It has been observed that the BN-Rd applied on both cell lines interacted with the cells at a concentration of 10 μg/ml and the cells radiated under a fluorescent microscope in the in vitro environment. In light of the findings obtained from this study, the potential of use in bio-reforming applications of bor nitride nanoparticles labeled with rodamin 123 floresan paint has been revealed.
The aim of this study is to label boron nitride (BN) nanoparticles with a fluorescent dye, rhodamine 123 (Rd), and to investigate its use for bioimaging in vitro condition. First, BN-Rd nanoparticles were produced by binding rhodamine 123 tag to boron nitride nanoparticles via glutaraldehyde crosslinker and characterized by SEM, UV-visible spectroscopy, MTT cell proliferation test, light microscope and fluorescence microscope analyses. BN-Rd nanoparticles at non-cytotoxic concentrations were applied on human prostate cancer cells (PC-3) and human embriyonic kidney cells (HEK-293) and the bioimaging potential of BN-Rd was investigated in vitro by examining the cells under fluorescence microscopy. As a result, BN and BN-Rd, which were determined to have a size of ~40 nm in SEM analysis, showed a cytotoxic effect at concentrations of 30 µg/ml and above. The nanoparticle-treated cell morphologies examined under a light microscope was found to support MTT results. It was observed that BN-Rd, at a concentration of 10 µg/ml applied on both cell lines interacted with the cells and the cells fluoresced under a fluorescence microscope in vitro. In the light of the findings obtained from this study, the potential use of boron nitride nanoparticles labeled with rhodamine 123 fluorescent dye in bioimaging applications has been revealed.
Alan : Mühendislik; Fen Bilimleri ve Matematik
Dergi Türü : Ulusal
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