Abstract:

In this study, previous studies have identified 10 isolates isolated from fermented products with the DuPont QualiconRiboPrintersystem.®Spectrophotometric and visual reading methods are determined.The highest activity was shown by the Lactobacillus curvatus P3X isolate.The approximately molecular weight of the enzyme of L. curvatusP3X is determined by the SDS-PAGE method.High tannaz activitesigösteren L. curvatus P3X's tannaz activity has been studied at different temperatures, pH, substrat intensity, tanic acid concentration and the effect of various minerals.The results of the tests were the optimal temperature 37°C, the concentration of methyl gallate used as pH 5.0 substrate was 7 mM, and the concentration of tannic acid was 1.75 mM. Lactic acid bacteria have reduced tanaz activity in the presence of Hg+2 and Mg+2 ions, while Ca+2 and Zn+2 have not been shown to affect tanaz activity. It has been found that the preferential active substance (Tween 80), shelate (EDTA), inhibitors (DMSO) and the substance that breaks the structure (Ore) are not associated with the activity of tannaz.

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