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Diyarbakır ili domates üretim alanlarında Domates sarı yaprak kıvırcıklık virüsü (Tomato yellow leaf curl virus, TYLCV), Patates Y virüsü (Potato virus Y, PVY), Domates mozaik virüsü (Tomato mosaic virus, ToMV), Domates lekeli solgunluk virüsü (Tomato spotted wilt virus, TSWV) ve Domates halkalı leke virüsü (Tomato ringspot virus, ToRSV)’nün varlığının moleküler yöntemler ile ortaya konulması amacıyla 2018 yılında sürvey çalışmaları yürütülmüştür. TYLCV’nin varlığını araştırmak için PCR yöntemi, PVY ve ToMV’nin varlığını araştırmak için multipleks RT-PCR yöntemi, TSWV ve ToRSV’nin varlığını araştırmak için ise RT-PCR yöntemi kullanılmıştır. Domates üretim alanlarında virüs belirtisi gösteren ve göstermeyen toplam 278 adet bitkiden rastgele yaprak örnekleri toplanmıştır. Moleküler yöntemler ile testlenen örneklerin 56 (%20.1)’sının en az bir virüs türü ile enfekteli olduğu tespit edilmiştir. Testlenen örneklerin 41’inin (%14.7) ToMV, 21’nin (%7.6) PVY ve 6’sının (%2.2) ise ToMV+PVY ile karışık enfekteli olduğu saptanmıştır. Çalışma kapsamında yürütülen moleküler testler sonucunda, domates örneklerinde TYLCV, TSWV ve ToRSV enfeksiyonları saptanmamıştır. Tespit edilen PVY ve ToMV izolatlarından rastgele 4’er adet seçilerek PVY izolatlarının kısmi kılıf protein genleri, ToMV izolatlarının kısmı hareket protein ve kılıf protein genleri karakterize edilerek, Gen Bankasına kayıtları yapılmıştır. ToMV izolatları dünyadaki diğer izolatlar ile nükleotid düzeyinde %99-100 arasında benzerlik gösterirken, PVY izolatları %88-99 arasında değişen oranda benzerlik göstermiştir. Yürütülen bu çalışma ile ToMV ve PVY Diyarbakır ili domates üretim alanlarında ilk defa rapor edilmiştir.
Tomato yellow leaf curl virus (TYLCV), Potato Y virus (Potato virus Y, PVY), Tomato mosaic virus (Tomato mosaic virus, ToMV), Tomato spotted wilt virus (TSWV) and Tomato ringspot virus (Tomato ringspot virus, ToRSV) have been carried out in 2018 with the aim of detecting the presence of tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV), the potato Y virus (Potato virus Y, PVY), the tomato mosaic virus (Tomato mosaic virus, ToMV), the tomato spotted wilt virus (Tomato spotted wilt virus, TSWV) and the tomato ringspot virus (Tomato ringspot virus, ToRSV). The PCR method was used to investigate the existence of TYLCV, the multiplex RT-PCR method to investigate the existence of PVY and ToMV, while the RT-PCR method was used to investigate the existence of TSWV and ToRSV. In the tomato production areas, random leaf samples have been collected from a total of 278 plants that show and do not show viral symptoms. 56 (20.1%) of the samples tested by molecular methods were found to be infected with at least one type of virus. 41 of the samples tested (14.7 percent) were ToMV, 21 percent (7.6 percent) PVY and 6 percent (2.2 percent) mixed with ToMV+PVY. Molecular tests conducted under the study showed that no TyLCV, TSWV and ToRSV infections were detected in tomato samples. The detected PVY and ToMV isolates were randomly selected 4 pieces of the PVY isolates by characterizing the partial clay protein genes, the part of the ToMV isolates by characterizing the movement protein and clay protein genes, recorded in the Gen Bank. ToMV isolates are similar to other isolates in the world at 99-100% levels of nucleotid, while PVY isolates are similar at 88-99%. This study was conducted with ToMV and PVY Diyarbakır or tomato production fields for the first time.
Surveys were conducted to investigate the presence of Tomato yellow leaf curl virus (TYLCV), Potato virus Y (PVY), Tomato mosaic virus (ToMV), Tomato spotted wilt virus (TSWV) and Tomato ringspot virus (ToRSV) in tomato production areas of Diyarbakır province by molecular methods in 2018. The following methods were implemented to detect the presence of the viruses: PCR method for TYLCV, multiplex-RT-PCR method for PVY and ToMV, and RT-PCR method for TSWV and ToRSV. A total of 278 symptomatic and asymptomatic leaf samples were randomly collected from tomato plants. Fifty-six (20.1%) samples tested by molecular methods were found to be infected with at least one virus. Of the samples tested, 41 were infected with ToMV (14.7%), 21 were infected with PVY (7.6%), and 6 contained mixed infections of ToMV + PVY (2.2%). TYLCV, TSWV, and ToRSV were not detected in the tomato samples tested. Four PVY and four ToMV isolates were randomly selected to characterize the partial movement protein (MP) and coat protein (CP) genes of ToMV, and the partial coat protein (CP) gene of PVY. The nucleotide sequences were deposited in the GenBank database. Diyarbakır isolates of ToMV showed 99-100% similarity while Diyarbakır isolates of PVY showed 88-99% similarity to the other isolates reported around the world. With this study, ToMV and PVY were reported for the first time in tomato production areas in Diyarbakır province.
Alan : Ziraat, Orman ve Su Ürünleri
Dergi Türü : Ulusal
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