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Bu çalışmada, Ağustos 2013-Şubat 2015 tarihleri arasında Afyonkarahisar’da tüketime sunulan 100’er adet yürek, taşlık ve ciğerden oluşan toplam 300 tavuk iç organ örneğinde, L. monocytogenes ve Salmonella türlerinin immunomagnetik seperasyon tekniği ile saptanması, izolatların antibiyotik duyarlılık profillerinin belirlenmesi amaçlanmıştır. İç organ örneklerinden %13 Salmonella spp. ve %2,6 L. monocytogenes saptandı. Elde edilen 39 Salmonella spp. izolatının 9’u yürekten (%23,2), 1’i taşlıktan (%2,5), 29’u (%74,3) karaciğerden izole edildi. L. monocytogenes’in iç organ çeşidine göre izolat sayıları sırasıyla; yürekten 2 (%25), taşıktan 5 (%62,5) ve karaciğerden 1 (%12,5) adet olarak belirlendi. Salmonella spp. izolatlarının %100’ünün tetrasiklin, klortetrasiklin ve oksitetrasiklin’e, %64,1’inin penisilin’e, %48,7’si ampisilin’e, %46,1’inin florfenikol’e, %43,5’inin amoksisilin’e, %26’sının streptomisin’e, %15,3’ünün enrofloksasin’e, %7,6’sının trimetoprim/sülfadiazin’e dirençli olduğu saptanırken, gentamisin dirençliliği gözlendi. Elde edilen L. monocytogenes izolatlarının %62,5’inin tetrasiklin, klortetrasiklin, oksitetrasiklin ve trimetoprim/sülfadiazin’e, %50’sinin amoksisilin’e, florfenikol’e, %37,5’inin enrofloksasin’e, %25’inin ampisilin ve penisilin’e, %12,5’inin streptomisin’e dirençli olduğu belirlendi. Salmonella spp. ve L. monocytogenes izolatlarının hiçbirinde gentamisin direnci saptanmadı. Salmonella spp. ve L. monocytogenes izolatlarının en az bir ve daha fazla antibiyotiğe çoklu dirençli oldukları belirlendi.
In this study, a total of 300 internal organs of chicken samples consisting of 100 pieces of heart, stone and liver presented for consumption in Afyonkarahisar between August and February 2015, the detection of L. monocytogenes and Salmonella species by immunomagnetic separation technique, the purpose of determining the antibiotic sensitivity profiles of the isolat. 13% of the internal organ samples are salmonella spp. And 2.6% L. monocytogenes was detected. It has 39 Salmonella spp. 9 of its isolates were isolated from the heart (23.2%, 1 from the stone (2,5%) and 29 (74.3%) from the liver. According to the internal organs of L. monocytogenes, the number of isolates was determined as 2 (25% of the heart), 5 (62.5%) and 1 (12.5%) of the liver, respectively. The Salmonella spp. Its isolates were 100% resistant to tetrasiclin, chloretrasiclin and oxythrasiclin, 64.1% resistant to penicillin, 48.7% resistant to ampicillin, 46.1% resistant to florfenicol, 43.5% resistant to amoxicillin, 26% resistant to streptomycin, 15.3% resistant to enrofloxacin, 7.6% resistant to trimetoprim/sulfadizin. 62.5 percent of the obtained L. monocytogenes is resistant to tetracycline, chlorethracycline, oxythracycline and trimetoprim/sulfadiazine, 50 percent resistant to amoxicilline, florfenicol, 37.5 percent resistant to enrofloxacin, 25 percent resistant to ampicilline and penicilline, 12.5 percent resistant to streptomycin. The Salmonella spp. And in none of the L. monocytogenes isolates the resistance of gentamisin was detected. The Salmonella spp. and L. The monocytogenes is found to be multi-resistant to at least one and more antibiotics.
This study consists of 100 hearts and gizzards offered for retailed in Afyonkarahisar between August 2013 and February 2015. It was aimed to determine L. monocytogenes and Salmonella species by immunomagnetic separation technique in a total of 300 chicken internal organs and to determine the antibiotic susceptibility profiles of the isolates. 13% (39/300) Salmonella spp. 2.6% L. monocytogenes were detected in internal organ samples. The 39 Salmonella spp. 9 of the isolates were isolated from the heart (23.2%), 1 from the gizzard (2.5%), 29 (74.3%) from the liver. According to the internal organ type of L. monocytogenes isolate numbers are; 2 (25%) from the heart, 5 (62.5%) from the stone, and 1 (12.5%) from the liver. Salmonella spp. 100% of its isolates are tetracycline, chlortetracycline, and oxytetracycline, 64.1% to penicillin, 48.7% to ampicillin, 46.1% to florphenicol, 43.5% to amoxicillin resistance was found to be 26% to streptomycin, 15.3% to enrofloxacin and 7.6% to trimethoprim/sulfadiazine, whereas resistance to gentamicin was observed. 62.5% of isolates to tetracycline, chlortetracycline, oxytetracycline and trimethoprim / sulfadiazine, 50% to amoxicillin, florphenicol, 37.5% to enrofloxacin, 25% to ampicillin and penicillin 12.5% were found to be resistant to streptomycin. Gentamicin resistance was not detected in any of the Salmonella spp. and L. monocytogenes isolates.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
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