Abstract:

Purpose: In cases of sepsis, death rates are increased per hour of loss in cases receiving or not receiving incorrect antimicrobial treatment. In this study, the aim is to compare the conventional blood culture/MALDI TOF method with the MALDI TOF MS (LFM) and the microarray method after lizis filtration in order to identify the factor faster than the conventional culture method. Method: between November 2015-February 2016 Ege University, Medical Faculty Hospital, Adult Intensive Care units taken from patients in blood culture samples of 39 grams of negative influencer microarray and LFM process was applied and then studied with the MALDI TOF system. The results of the MALDI TOF and microarray testing methods applied after lizis filtration were compared with the MALDI TOF type-level identification made from the bacterial colony. Results: The positive signal time in the blood culture device was set as short as seven hours 55 minutes, the longest 31 hours 22 minutes, the average positive time 12 hours 55 minutes. The post-cultural MALDI TOF identification and LFM identification sensitivity were calculated at 82% (32/39), while the microarray method sensitivity was calculated at 87.1%. The likelihood of the MALDI TOF and microarray methods applied after Lizis filtration was 79.4 % (31/39), while the proportion of samples given consistent results by both three methods was 76.9 % (30/39) calculated. The three most frequently detected factors are Klebsiella spp, Acinetobacter spp. and Escherichia spp. Compatibility with the post-cultural MALDI TOF and LFM method was calculated as 0.715, 0.843, 0.938; Compatibility with the microarray method was calculated as 0.935, 0.753, 0.938 and a high compatibility was achieved for both three bacteria. The result: Based on the obtained data, it is believed that both lizis filtration and microarray platforms are useful in order to shorten sepsis diagnosis. The Lizis filtration method has been leading in terms of cost efficiency.

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