Özet:

e: MRSA poses a great risk to wound patients with potential to cause significant mortality and morbidity in human population due to the development of biofilm. So the present study was undertaken with the aim to know the prevalence of different microorganism from wound, to know the biofilm formation and the antimicrobial resistant pattern of MRSA strains. Materials & Methods: The prospective study was carried out in the department of Microbiology during the period of July 2012 to December 2013.Total of 85 MRSA isolated from clinical samples were identified by standard microbiological techniques and the isolates were further tested for biofilm formation and Antimicrobial susceptibility testing by Kirby-Bauer disc-diffusion method as per CLSI guidelines. Result: Among 290 wound sample received, culture was positive in 230 (79.31%). The common pathogens were MRSA 85(32.44%), MSSA 60(22.90%), E.coli 25(9.54%) and Pseudomonas aeruginosa 24(9.16%). Higher prevalence was noted in Obgy (38.82%) followed by surgery (17.65%), Skin (16.47%), ICU(9.41%), Orthopedics(7.06%), Medicine(4.71%), OPD(3.53%), Paediatric (2.35%),Of 85 MRSA, biofilm formation was observed in 70 (82.35%). Strong biofilm formation in 42(49.41%), weak biofilm formation in 28(32.94%) and negative biofilm formation in 15(17.65%). In our study the Antimicrobial resistance pattern in strong biofilm forming MRSA isolates when compared to biofilm non producers was for Amikacin 57.14%/ 26.67%, Erythromycin 73.80%/ 26.67%, Clindamycin 66.67%/ 20%, Ciprofloxacin 90.47%/46.67%, Gentamycin 88.09%/46.67%, Fusidic acid 35.71%/6.67%, Mupirocin 14.28%/6.67%.No resistance was noted to Linezolid and Teicoplanin Conclusions: Early identification and adopting efficient control protocol against biofilm forming MRSA can be one of the essential steps towards the prevention of the most serious nosocomial infections.

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