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A new reversed-phase liquid chromatographic (RPLC) method for the determination of methyl salicylate in a medicated cream formulation was developed and validated. The separation was achieved using an isocratic mobile phase, on a Lichrosorb C8 column. The eluent was monitored by photodiode array detection at 304 nm. The calibration curve showed excellent linearity (R2 = 0.9999) over the concentration range of 25-175 ug/mL. The recovery of methyl salicylate was in the range from 99.78100.0%. The percent relative standard deviation values for intra- and inter-day precision studies were <2.0%. The method is very simple, sensitive and robust with short runtime (<3.0 min) to enable the processing of numerous quality control samples.
Field : Sağlık Bilimleri
Journal Type : Uluslararası
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