Özet:

Diabetic nefropathy is one of the major complications of diabetes mellitus. Nitric oxide (NO) is a multifunctional mediator that is effective in hemodynamic changes in diabetes. Poly ADP ribose polymerase (PARP) is an enzyme that plays a role in repairing DNA throughout the apoptotic process. The proteolithic destruction of this enzyme prevents DNA repair and causes apoptosis. Excessive activation results in apoptosis. In this study, it was intended to study the inducable nitric oxide sentase (iNOS) and PARP immune reactiveness in the rats' kidney tissue created by experimental diabetes with streptosotine (STZ). Method: In the study, 14 adult Wistar Albino male rats were used. The rats were divided into 2 groups: Group I (Control) and Group II (Diabetes). A single dose of 60 mg/kg of STZ was administered intraperitoneally in a 0.1 M phosphate citrate tampon (pH: 4.5). The rats of all groups were decaptivated under anesthesia at the end of the 10 weeks of experiment, kidney tissues were removed, and 10 percent formaldehyde for light microscopic examination, paraffin blocks were prepared. In 5 μm paraffin cuts, the immunreactivity of iNOS and PARP was monitored by the method of avidin-biotin peroxidase. The severity and frequency of immunoreactivity was scored from 0 to +3 and evaluated as semi-quantitative. Results: in the light microscopic examination of immunohistochemical painting performed to determine the expressions of iNOS and PARP; in the control group, the immunoreactivity of iNOS and PARP was quite intense, compared to the control group, while in the diabetic group, the immunoreactivity of iNOS and PARP was quite intense, and the intensity and prevalence of +3 were confirmed. The result: With this study, the diabetic group has achieved a significant contribution to the damage in the kidney tissues by the iNOS and PARP, which are effective in the apoptosis process.

Anahtar Kelimeler: