Abstract:

Stachys is one of the widespread species in the Lamiaceae family. Species of this species are known for their biological activity and ethnomedical uses. In this study, the total phenolic and flavonoid content of extracts obtained by different solvents (etanol, methanol and ethyl acetate) of the surface parts of Stachys macrantha and the activity of antioxidants (ABTS, DPPH) and enzyme inhibition (α-amilase, α-glukozidase, butirylcolinesterase and acetylcolinesterase) was studied by spectrophotometric methods. Also, the phenolic component analysis has been analyzed with HPLC. The findings have found that methanol extract has a remarkable biological activity compared to other extracts. Extract, total phenolic (45.57 mg GAE/g extract) and flavonoids (54. The content of 48 mg QE/g extract, antioxidant properties [ABTS (49.99 mg TE/g extract), DPPH (23.62 mg TE/g extract)] and enzyme inhibition activities [α-amilase (159.35 mmol ACE/g extract), α-glukozidase (8.78 mmol ACE/g extract), butirylcolinesterase (5.85 mmol ACE/g extract) and acetylcolinesterase (1.25 mmol ACE/g extract)] have been determined. The major phenolic components of methanol extract have been found to be kaemferol (539.65 μg/g extract) and curcetin (271.65 μg/g extract). According to the results obtained, it can be said that the plant has the potential to be used in the pharmaceutical, food and cosmetic industries thanks to its detected activities. However, the data in question must be supported by further studies and the effects of the active components by purification in vivo environment must be determined.

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