Özet:

Objective: The aim of this study is to investigate the changes of temporomandibular joint (TMJ) by experimentally induced osteoarthritis (OA) and the effectiveness of botulinum toxin A (BTX-A) treatment via histopathological and biochemical methods. Methods: Sprague-Dawley rats, aged 8 to 10 weeks, were used in this study. Intra-articular monosodium iodoacetate (MIA) solution was injected bilaterally into two experimental groups to induce TMJ osteoarthritis. Sterile 0.9% NaCl solution was injected to TMJ of healthy control group bilaterally to investigate the effect of trauma by intra-articular injection. Development of experimental osteoarthritis took 4 weeks, and blood samples were harvested from MIA group and examined to prove the presence of induced osteoarthritis. OA-induced eight rats with BTX-A. Another OA-induced eight rats were classified as a control group and intra-articular 0.9% NaCl solution was injected bilaterally. All samples were sacrificed 6 weeks after treatment. Blood samples and specimen of TMJ were harvested from all rats and examined histopathologically and biochemically. Results: BTX-A and 0.9% NaCl solution groups showed some improvement compared with untreated MIA group but it was not statistically significant. There was no sign of trauma in 0.9% NaCl solution-injected healthy group. Pathological findings were also consistent with the biochemical results. Conclusion: BTX-A was not successful at treatment of OA and is not a proper selection especially when used intra-articularly.

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