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SuleymanDemirel University TIP FACULTY JOURNAL: December 2005; 12(4) Cyclosporin A has an important use in the prevention of the breakthrough of cyclosporin A (CsA) in organ transfusions, assessing the effects of Vitamin C and Vitamin E and verapamilin in the light microscope. This strong immune system is the important side effect of the agent that oppresses the nefrotoxicides. The two important mechanisms accused of the formation of this side effect are: i) vasoconstructions in the renal afferent arteriol ii) the effect on the enzymes of the antioxidant system increases the free oxygen radicals. The CSA applied to the antioxidant properties of Vit E and Vitamin C, by blocking the calcium channels and giving verapamili showing vasodilatator activity, we did this study in order to evaluate the histopathological changes in the kidney cartilage in different experimental groups and to investigate the effects of these changes on the antioxidant and vasodilatator agents given in one and together. Material and Method: 25 rats; the control group was divided into five groups, including the group given cyclosporin A (CsA), the group given CsA+ vitamins E and C, the group given CsA+verapamil and the group given CsA+vitamin+verapamil. For 10 days, CsA was given in oral dose of 50 mg/kg with nazogastric sonda, vitamin E in 150 mg/kg in intramuscular dose; vitamin C in 200 mg/kg in intraperitoneal dose, verapamil in 0.5 mg/kg in intramuscular dose. At the end of the study, the blood and kidney tissues of rats were taken under anesthesia. The kidney tissue was detected in a 10% formula. With routine histological follow-up, 5ï­m cuts were taken from the blocked tissues and evaluated by painting with hematoxylene-eosine. Results: At the end of our study, histologically significant structural changes occurred in the CsA given group. These changes were partially improved in CsA + vitamin E and C given group and CsA + verapamil given group; normal histological structure appeared in CsA + vitamin + verapamil field group. Furthermore, antioxidant enzymes in kidney tissue from glutathion proxidase and catalazdabelirgin increase and kidney function tests from BUN and creatin debelirgin improvement were also observed in this group. The result: For the prevention of the nephrotoxycin by CsA, we believe that the use of verapamil, vitamin E and C together will be more beneficial. Keywords: Cyclosporin A, Nephrotoxicity, Verapamil, Vitamin E, Vitamin C Abstract Effects of Vitamin C, Vitamin E and Verapamil on cyclosporin induced nephrotoxicity in rats: a light microscobic study Aim: Cyclosporin A (CsA) is an important agent used in organ transplantation to prevent rejection. This immunosuppressive drug's most important secondary effect is nephrotoxicity. There are two hypotheses for the nephrotoxicity mechanism i) vasoconstruction in afferent arteriole ii) the role of reactive oxygen species. In this study we planned to see the individual or combined effects of antioxidant vitamins E and C with a calcium channel blocker verapamil. Materials and Methods: 25 rats divided into five groups equally; a vehicle group, CsA treated group, CsA + vitamin E and C treated group, CsA + verapamil treated group and CsA + vitamin E and C + verapamil treated group. CsA administered 50 mg/kg/day (per oral by catheter), vitamin E administered 150 mg/kg/day (intramuscular), vitamin C administered 200 mg/kg/day (intraperitoneal), verapamil administered 0.5 mg/kg/day (intramuscular) for 10 days. At the end of the study rats' blood samples and kidney tissues were taken under ether anesthesia. Tissue sections of kidney were fixed in 10% formalin. Parafin sections were cut at 5 ï­m, then examined after staining with hematoxylin-eosin. Results: In CsA treated rats, significant morphological changes were observed in kidney sections. Although in CsA + vitamin E,C and CsA + verapamil treated rats, these changes were reduced but the most significant improvement observed in the CsA + vitamin C and E + verapamil treated group. Also most significant increase of antioxidant enzymes, catalase and glutathion peroxidase in renal tissue was seen in this group. Conclusion: We suggest that using the combination of verapamil, vitamin E and C will be more useful to prevent the nephrotoxicity caused by CsA. Key words: Cyclosporin A, Nephrotoxicity, Verapamil, Vitamin E, Vitamin C

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