Özet:

Background: The continuous emergence of multi drug resistant bacteria severely reduces the effectiveness of antibiotics increases the frequency of therapeutic failure. In recent years, researchers often prefer natural herbal resources for the discovery of new antibacterial drugs. In the world, Artemisia L. genus, are the well-known species of the family Asteraceae, have botanical and pharmaceutical attention due to their characteristic smells or flavors. Objective: The present study researches the antibacterial activities against carbapenem resistant bacteria of Artemisia absinthium L., A. ludoviciana Nutt. and A. vulgaris L. Method: Methanol extracts and essential oils were prepared at concentrations of 250, 500, 1000, 2000 μg/mL; 15.625, 31.25, 62.5, 125 μg/mL respectively. Broth microdilution assay was used to determine minimum inhibitory concentrations (MICs) of samples against some Gram negative standard strains (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 700630, Acinetobacter baumannii ATCC 19606) and clinically carbapenem resistant Gram negative bacteria strains (E. coli, K. pneumoniae, P. aeruginosa, Acinetobacter spp.). Results: A. absinthium essential oil had antibacterial effect on standards and clinical strains (15.625-125μg/mL) except carbapenem resistant P. aeruginosa strain. In addition, A. absinthium essential oil was detected antibacterial effect against clinical Acinetobacter spp. at very low (31.25 μg/mL) concentration. A. ludoviciana essential oil was effective against P. aeruginosa and A. Baumannii strains at 15.625 and 125 μg/mL respectively. Conclusion: Artemisia species may be significant inhibitors of carbapenem resistant Gram negative strains.

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