Özet:

Rubus idaeus L. is one of the oldest and most widespread berry crops, which is cultivated for the excellent taste as well as for medical and dietary properties of berries. Rubus fruits contain a significant amount of vitamins A and C, anthocyanins, polyphenolic substances, which determines their high antioxidant activity. Remontant red raspberry forms are able to berry on annual shoots in the second half of summer, which extends the term of consumption of fresh berries by 1.5-2 months. However, many forms of remontant raspberry have a low potential for vegetative propagation compared to summer varieties, which makes them difficult to reproduce and to use in the breeding process. We investigated the possibility of increasing the efficiency of in vitro micropropagation of a remontant raspberry variety ?Biryulevskaya’. The effects of 6-benzylaminopurine (6-BAP) at concentrations of 0.5–3 mg l-1 and thidiazuron (TDZ) at concentrations of 0.05–0.2 mg l-1 as well as doubled and tripled iron chelate Fe-EDTA (Ferric ethylenediamine-tetraacetic acid) doses were studied. We found the adding 1.0 mg l-1 6-BAP to the MS medium containing a triple dose of iron chelate, provided intensive proliferation of high quality adventitious shoots.  Keywords: Red raspberry; Remontant form; In vitro reproduction; Plant growth regulators; Iron chelate References Anderson, W. C. (1980). Tissue culture propagation of red raspberries and black raspberries, Rubus idaeus and R. occidentalis. Acta Hort., 112, 13-20. Borodaeva, Zh. A., Muratova, S. A., Kulko, S. V., & Tokhtar, L. A. (2017). Influence of various sources of carbohydrate nutrition on rhizogenesis of microcrops of berry crops under the in vitro conditions. Belgorod State University Scientific Bulletin. Biological sciences, 41, 25 (274), 21-35. 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E., Frei, B., & Wrolstad, R. E. (2002) Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, Rubus and Ribes. Journal of Agricultural and Food Chemistry, 50(3), 519-525. DOI: 10.1021/jf011062r Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiol. Plant, 15, 473-497. Poothong, S., & Reed B. M. (2015). Increased CaCl2, MgSO4, and KH2PO4 improve the growth of micropropagated red raspberries. In Vitro Cell. Dev. Biol. – Plant, 51, 648-658 DOI 10.1007/s11627-015-9720-y Poothong, S., & Reed, B. M. (2014). Modeling the effects of mineral nutrition for improving growth and development of micropropagated red raspberries. Scientia Horticulturae, 165, 132-141. doi:10.1016/j.scienta.2013.10.040 Shornikov, D. G., Bryukhina, S. A., Muratova, S. A., Yankovskaya, M. B., & Papikhin, R. V. (2010). In vitro conditions improvement for berry and ornamental plants micropropagation. 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Virus infections reduce in vitro multiplication of ‘Malling Landmark’ raspberry. In Vitro Cellular & Developmental Biology. – Plant, 36. 65-68. 10.1007/s11627-000-0015-5. Tsao, C. W. V., Reed, B. M. (2002). Gelling agents, silver nitrate, and sequestrene iron influence adventitious shoot and callus formation from Rubus leaves. In Vitro Cell. Dev. Biol. – Plant, 38, 29-32. Wu, J. H., Miller, S. A., Hall, H. K., & Mooney, P. A. (2009) Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell, Tissue and Organ Culture, 99 (1). 17-25. DOI: 10.1007/s11240-009-9571-5 Xiao, D., Huang, Y., Park, E., Edirisinghe, I., & Burton-Freeman, B. (2017). Red raspberries and insulin action: understanding the role of red raspberry consumption on postprandial metabolic indices. FASEB Journal, Bethesda, 31(1) 973-979. Zawadska, M., & Orlikowska, T. (2006a). Factors modifying regeneration in vitro of adventitious shoots in five red raspberry cultivars. 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