Abstract:

Purpose: This study aims to identify the heterogenity and distribution of the mast cells located in the different regions of the genital canal in the pre- and post puberty periods in female blisters (Coturnix coturnix japonica) with different detection solutions and coloring methods. In the study, 10 tissue samples taken from the genital channel of healthy female bladder (infundibulum, magnum, istmus, uterus, vagina) were used as material in the period before puberty and 10 after puberty. Some of the tissue samples taken from both groups were detected in a 10% neutral formaldehyde and some in the isotonic formaldehyde acetic acid detection solution. The cuts taken from the tissues were applied to Toluidin blue and alcian blue/safranin O coloring methods. Results: When prepared preparations were studied in the light microscope in both detected solutions, round, oval or macic-shaped mast cells were observed in the layers of the genital canal's tunika mucosa, tunika muskularis and tunika seroza, especially located around the blood vessels. It was observed that the muscle cells were most placed in the uterus both before and after puberty; then, respectively, it showed increasingly decreased intensity in the istmus, magnum, vagina and infundibulum. Mast cells in the infundibulum were found to increase significantly (p<0,05) compared to the pre-puberty period in the post-puberty period. In all the tissues identified by IFAA, the mast cells were colored more clearly and in numeric assessment were 10 percent more than the neutral formaldehyde detected. Recommendation: As a result, the number of mast cells in the infundibulum increased, in the post-puberty period; this increase could have an effect on the formation of eggs and ovulation.

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