Özet:

Staphylococcus aureus (S.aureus) can cause serious fatal infections due to several enzymes and toxins that it secretes when it encounters favorable conditions.For this reason, S.aureus is one of the most important pathogens currently causing hospital and community infections.Methicillin-resistant S.aureus(MRSA) strains are resistant to many antibiotics and have the ability to rapidly develop resistance to currently used antibiotics during treatment. MRSA origins cause serious infections with high mortality and also increase the rate of hospital infections that require difficult and longer hospitalization and the use of greater quantities of antibiotics in risk-prone units in hospitals. Another factor that is as important as MRSA in S.aureus pathogenicity is the Panton-Valentine leukocidin(PVL) toxin secreted by S.aureus strains, most commonly isolated from deadly necrotizing pneumonias and community-acquired soft skin tissue infections.PVL is especially observed in community-acquired S.aureus strains. However, studies showed that PVL-positive origins began to spread rapidly to hospital environments. In this study, methicillin-sensitive S.aureus, methicillin-resistant coagulase-negative Staphylococcus (MR-CNS), and Panton-Valentine leukocidine genes were identified by real-time polymerase chain reaction (RT-PCR) by investigating the rate of nasal S.aureus carriers(NSAT) in nasal swab samples taken from hospital staff belonging to the highest risk group for staphylococcal infections, at which screening is aimed. Classical NSAT scans were performed on nasal swap samples taken from 98 healthcare professionals in Hospital. Nasal S.aureus (4 MSSA,13 MRSA) was detected in 17 samples(17.35%). During detection, 48 MR-CNS were detected. In addition, 61 mecA (+),17 nuc (+),1 pvl (+) genes were detected in RT-PCR samples directly from the nasal swabs taken in accordance with the culture method.