Different rootstock and scion combinations have different effects on all agronomical and physiological parameters. Determining good affinity, optimizing environmental factors and building successful graft union are very important to obtain successful graft combination and healthy plants. This study was conducted in Manisa Viticultural Research Station in 2014. Crimson Seedless, Cardinal and Michele Palieri grape varieties and 1103 Paulsen (Vitis berlandieri x Vitis rupestris) and Kober 5 BB (Vitis berlandieri x Vitis riparia) rootstocks were used as plant materials in this study. Grafts were made by using “omega” graft machine. Data were analyzed into two stages; at the end of callusing phase and sapling production phase. Grafts were placed into callusing room (26 0C and 90-95% relative humidity) for 24 days and callusing levels , sprouting and rooting ratios (%) were determined at the end of this period. Grafts were rooted under controlled conditions (26±2 0C and 70-75% relative humidity) for six weeks then were transferred shading area for adaptation. Callusing levels , sprouting and rooting ratios (%), shoot development level , main shoot length (cm) and diameters (mm), number of nodes on main shoot, chlorophyll content and ratio of grafts were recorded and analyzed statistically . The best result was obtained from the combinations of ‘Cardinal/5 BB (80.00%)’ and ‘Michele Palieri/1103 P (78.33%)’ in respect to graft ratio. The lowest graft ratio were obtained ‘Crimson Seedless/1103P (46.66%)’ combination
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